Fig. 1: The effect of activin-A on the luteal steroidogenic function and JNK expression in human luteal granulosa cells. | Cell Death Discovery

Fig. 1: The effect of activin-A on the luteal steroidogenic function and JNK expression in human luteal granulosa cells.

From: Terminal differentiation of human granulosa cells as luteinization is reversed by activin-A through silencing of Jnk pathway

Fig. 1: The effect of activin-A on the luteal steroidogenic function and JNK expression in human luteal granulosa cells.

Activin-A treatment is associated with significant decrease in the expression of steroidogenic enzymes StAR, P450 SCC, 3β-HSD, and 17β-HSD on qRT-PCR (a) and western blotting (b, c) along with decreased production of E2 and P4 (d). Note that aromatase expression did not change meaningfully after activin-A treatment either at transcriptional or translational level. The expression of phospho-c-JunSer73 and phospho-JNKSerThr183/Tyr185 expression were gradually diminished along with the steroidogenic enzymes in western blotting (b, c). FSH receptor expression was significantly upregulated and LH receptor was down-regulated in the cells in a dose-dependent fashion after activin-A treatment on qRT-PCR (a).

Back to article page