Fig. 2: Nomination of putative mitophagy regulatory pathways.
From: MEKK3-MEK5-ERK5 signaling promotes mitochondrial degradation
a Candidate mitophagy-regulating genes identified by FuSiOn were screened by siRNA-mediated depletion in U2OS mito-mCherry cells and U2OS GFP-LC3B cells. Red dots denote selectivity of candidate gene for mitochondrial degradation. b Model of the MAP3K3 kinase cascade including inhibitors. c Protein levels of p62 and mitochondrial marker TOMM40 from mouse embryonic fibroblasts treated with vehicle (DMSO) or the indicated concentrations of the BIX02189 overnight. d Mitochondrial accumulation in U2OS mito-mCherry cells treated overnight with vehicle (DMSO) or BIX02189 was measured by flow cytometry. Mean ± SD of n = 2 independent experiments is shown. e Mitochondrial accumulation in U2OS mito-mCherry cells treated overnight with vehicle (DMSO) or BIX02188 was measured by flow cytometry. Mean ± SD of n = 3 independent experiments is shown.
