Fig. 5: Sempervirine is a fluorescent molecule and preferentially binds to rRNA.
From: Sempervirine inhibits RNA polymerase I transcription independently from p53 in tumor cells
A Fluorescence and light microscopy images of in vivo labeled NCCIT cells after 1, 6, and 24 h of incubation with 5 μM sempervirine. Scale bar = 20 μM. n = 3, 60 inspected fields. B Fluorescence images of fixed/permeabilized control NCCIT cells treated with or without 100 μg/ml RNAseA (15 min), and stained with sempervirine and Draq5 as nuclear counterstain. Scale bar = 20 μM. n = 2, 40 inspected fields.
