Table 2 Antibodies list and dilution.
From: Sempervirine inhibits RNA polymerase I transcription independently from p53 in tumor cells
Antibody name | Specification | Dilution |
|---|---|---|
RPA194 | sc-48385 (Santa Cruz Technologies) | 1:1000–1:200 |
P53 | sc-126 (Santa Cruz Technologies) | 1:1000 |
MDM2 | OP46 (Sigma-Aldrich) | 1:500 |
UBF-1 | sc-13125 (Santa Cruz Technologies) | 1:1000 |
GAPDH | Ab9485 (Abcam) | 1:2000 |
Tubulin | #T4026 (Sigma-Aldrich) | 1:2000 |
Nucleolin | sc-8031 (Santa Cruz Technologies) | 1:1000–1:200 |
Fibrillarin | sc-11336 (Santa Cruz Technologies) | 1:1000 |
E2F1 | sc-251 (Santa Cruz Technologies) | 1:1000 |
BrdU | sc-32323 (Santa Cruz Technologies) | 1:1000 |
PARP | sc-7150 (Santa Cruz Technologies) | 1:1000 |
P63 | Generous gift of Prof. A. Peschiaroli | 1:1000 |
P73 | Generous gift of Prof. A. Peschiaroli | 1:1000 |
Rb | Sc-50 (Santa Cruz Technologies) | 1:1000 |
RAD51 | sc-9349 (Santa Cruz Technologies) | 1:1000 |
RNA pol II | sc-899 (Santa Cruz Technologies) | 1:1000 |
Clathrin | Ab-2731 (Abcam) | 1:1000 |
γH2AX | #9718 (Cell Signaling Technologies) | 1:500 |
S6 | #2217 (Cell Signaling Technologies) | 1:1000 |
Phospho-P53 | #9284 (Cell Signaling Technologies) | 1:1000 |
P21 | sc-6246 (Santa Cruz Technologies) | 1:1000 |
