Fig. 2: ADO drives the endogenous synthesis of hypotaurine in glioma.
From: ADO/hypotaurine: a novel metabolic pathway contributing to glioblastoma development
a ADO knockout cells were generated by utilizing CRISPR-Cas9 system in LN229. b Clone formation assays were conducted to evaluate the effect of ADO-Cas9 and hypotaurine on LN229 growth. c, d Serial sections or tumor tissue lysates of one patient were used to detect ADO and CDO expression by immunohistochemistry (IHC) and western blot, respectively. Scale bars = 50 μm. e Immunoblotting analysis of the CDO protein levels in U118 ADO-OE cells and f LN229 ADO-Cas9 cells. All results are representative of three independent experiments.
