Fig. 3: Inhibition of miR-21 exacerbated ischemic AKI. | Cell Death Discovery

Fig. 3: Inhibition of miR-21 exacerbated ischemic AKI.

From: LncRNA GAS5 promotes apoptosis as a competing endogenous RNA for miR-21 via thrombospondin 1 in ischemic AKI

Fig. 3

a Schematic diagram depicting the animal treatment procedure. b Knockdown of miR-21 in mice exposed to delayed IPC remarkably increased serum creatinine levels (N = 6). c Representative images of renal H&E (×10 magnification) and TUNEL staining (×20 magnification). Histopathological scores (d) (N = 6) and apoptotic rates (e) (N = 3) were higher in the anti-miR-21+IPC+I/R group than those in the anti-scramble+IPC+I/R group. f Inhibition of miR-21 significantly increased GAS5 and TSP-1 mRNA levels (N = 6). g Knockdown of miR-21 upregulated TSP-1 protein expression (N = 4). *P < 0.05, **P < 0.01 vs. anti-scramble+IPC+I/R group. Scale bars, 100 μm.

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