Fig. 4: miR-21 targeted TSP-1 might contribute to H/R or renal I/R treatment-induced apoptosis. | Cell Death Discovery

Fig. 4: miR-21 targeted TSP-1 might contribute to H/R or renal I/R treatment-induced apoptosis.

From: LncRNA GAS5 promotes apoptosis as a competing endogenous RNA for miR-21 via thrombospondin 1 in ischemic AKI

Fig. 4

a Luciferase reporter assays confirmed an interaction between miR-21 and the 3′-UTR of TSP-1 mRNA. N = 7, *P < 0.05 vs. anti-scramble+TSP-1 3′UTR. miR-21 was upregulated (b), and the TSP-1 protein was downregulated (c) by H6R0.5 treatment in HK-2 cells, N = 3, &P < 0.05, &&P < 0.01 vs. the Control. Effective knockdown of miR-21 by LNA anti-miR-21 (d) exacerbated H6R0.5-induced apoptosis (e, f) and increased the protein expression of TSP-1 (g), N = 3, #P < 0.05, ##P < 0.01 vs. anti-scramble+H6R0.5. h, i Serum creatinine levels were lower in miR-21+/0 mice with higher miR-21 levels (N ≥ 4). j–m miR-21+/0 mice presented lower histopathological scores, lower apoptotic rates and downregulated TSP-1 protein expression compared with miR-21 0/0 mice (N = 2–4), $P < 0.05, $$P < 0.01 vs. the miR-21 0/0 group. Scale bars, 100 μm.

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