Fig. 4: Suppression of ATG7-dependent autophagy leads to premature senescence of NHEM under oxidative stress. | Cell Death Discovery

Fig. 4: Suppression of ATG7-dependent autophagy leads to premature senescence of NHEM under oxidative stress.

From: Dysfunction of ATG7-dependent autophagy dysregulates the antioxidant response and contributes to oxidative stress-induced biological impairments in human epidermal melanocytes

Fig. 4: Suppression of ATG7-dependent autophagy leads to premature senescence of NHEM under oxidative stress.

a Representative images of NHEM, transfected with control shRNA, ATG7 shRNA, empty vector, or lentivirus ATG7 at the third passage, which were treated with or without H2O2 or UVB. Scale bar = 100 μm. b Senescence-associated β-galactosidase (SA β-Gal) staining of NHEM transfected with control shRNA, ATG7 shRNA, empty vector, or lentivirus ATG7, which were treated with or without H2O2 or UVB. Scale bar = 500 μm. c Percentage of SA β-Gal-positive cells in NHEM transfected with control shRNA, ATG7 shRNA, empty vector, or lentivirus ATG7 treated with or without H2O2 or UVB. d Relative mRNA expression of p16 in NHEM transfected with control shRNA, ATG7 shRNA, empty vector, or lentivirus ATG7. Percentage SD from three independent experiments. *P < 0.05, **P < 0.01, ns, nonsignificant.

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