Fig. 2: IK depletion inactivates the DNA damage-induced ATM signaling pathway via decrease in the ATM level. | Cell Death Discovery

Fig. 2: IK depletion inactivates the DNA damage-induced ATM signaling pathway via decrease in the ATM level.

From: Deubiquitinase USP47-stabilized splicing factor IK regulates the splicing of ATM pre-mRNA

Fig. 2: IK depletion inactivates the DNA damage-induced ATM signaling pathway via decrease in the ATM level.The alternative text for this image may have been generated using AI.

a Cells transfected with siIK #1 for 47 h were treated with neocarzinostatin (NCS) at the indicated concentrations for an additional 1 h, and the levels of IK, pATM S1981, pATR S428, CHK1, CHK2, pCHK1 S345, pCHK1 S317, and pCHK2 T68 were examined. b Cells transfected with human IK or mouse IK for 18 h were transfected with siIK #1 for an additional 48 h, and the levels of IK and ATM were examined. c Cells transfected with siIK #1 for 24 h were treated with BrdU at 100 μM for an additional 24 h and stained with an anti-BrdU antibody. Representative images obtained by confocal laser microscopy were examined. The percentages of cells exhibiting more than 15 BrdU foci per nucleus (n > 162) are graphed. ***p < 0.001. d Cells transfected with siIK #1 for 48 h were treated with 10 μM camptothecin (CPT) or etoposide (ETP) for an additional 24 h. The cell growth was monitored for an additional 24 h using an IncuCyte live-cell imaging system. n = 3.

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