Fig. 5: ERK1/2 pathway was involved in DCA-TGR- induced HNF4α and following metaplasia markers expression.
From: TGR5-HNF4α axis contributes to bile acid-induced gastric intestinal metaplasia markers expression
a GES-1 cells were treated with DCA (0, 25, 50, 100, 150, and 200 μM) for 24 h. Then, MAPK and PI3K/Akt pathways were examined by WB. b GES-1 cells were pretreated with U0126 (10 μM), MK2206 (10 μM), and SB239063 (10 μM) for 1 h following DCA (200 μM) treatment for 24 h. Then, HNF4α expression was examined by WB. c GES-1 cells were pretreated with U0126 (10 μM) for 1 h following Deoxycholic acid (DCA) (150 and 200 μM) treatment for 24 h. Then p-ERK1/2, KLF4, and CDX2 proteins expression were examined by western blotting. d Further, TGR5 was knocked down by transfection with siRNA in GES-1 cells. Next, the cells were treated with DCA (200 μM) for 24 h. p-ERK1/2 and HNF4α were examined by WB.
