Fig. 2: Expression levels of glucose metabolism-related enzymes, products and PD-L1 under different intervention conditions. | Cell Death Discovery

Fig. 2: Expression levels of glucose metabolism-related enzymes, products and PD-L1 under different intervention conditions.

From: Glucose metabolism involved in PD-L1-mediated immune escape in the malignant kidney tumour microenvironment

Fig. 2

A The expression of PD-L1 in the 786-O and OS-RC-2 cell lines was detected by western blotting after 12 or 24 h of culture under low-glucose conditions. B The expression of PD-L1 in the 786-O and OS-RC-2 cell lines was detected by RT-qPCR after 12 or 24 h of culture under low-glucose conditions. C Western blotting was used to detect changes in PD-L1 expression in the 786-O and OS-RC-2 cell lines after 12 h of low-glucose culture followed by culture medium replacement with that of normal glucose concentration. D Lactic acid (LD) production in the two cell lines were different under low-glucose conditions. E Western blotting was used to detect the expression differences in HIF-1α, PFKFB3 and LDHA, key enzymes in glycolysis. Independent experiments were performed in triplicate. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

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