Fig. 7: Difference in IFN and PD-L1 expression levels during CD3 and renal cancer cell co-culture under different intervention conditions and integration of the whole mechanism pathway. | Cell Death Discovery

Fig. 7: Difference in IFN and PD-L1 expression levels during CD3 and renal cancer cell co-culture under different intervention conditions and integration of the whole mechanism pathway.

From: Glucose metabolism involved in PD-L1-mediated immune escape in the malignant kidney tumour microenvironment

Fig. 7

A The purity of T cells was confirmed by flow cytometry using an anti-CD3 FITC-conjugated antibody compared with a mouse isotype control antibody. B Protein levels of PD-L1 in 786-O and OS-RC-2 cells cultured in normal culture medium, glucose-free culture medium and glucose-free culture medium with PX-478 (40 mmol/L), YC-1 (10 mmol/L) and PFK-015 (10 mmol/L) for 24 h are shown. C After the two tumour cell lines were cultured alone under various conditions for 24 h and then cocultured with CD3+ lymphocytes for another 24 h under normal RPMI 1640 medium conditions, the expression level of IFN-γ in the supernatant of the culture system was detected by ELISA. D The pathway diagram summarises the relationship between glycolysis and its associated proteins and PD-L1. Independent experiments were performed in triplicate. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

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