Fig. 1: Experimental design and characteristics of primary culture of microglia and 3 lots of MSC. | Cell Death Discovery

Fig. 1: Experimental design and characteristics of primary culture of microglia and 3 lots of MSC.

From: Umbilical cord-derived mesenchymal stromal cells immunomodulate and restore actin dynamics and phagocytosis of LPS-activated microglia via PI3K/Akt/Rho GTPase pathway

Fig. 1

In this experiment, microglia were stimulated with LPS for 6 h and backed to culture in NM. In LPS + MSC group, co-cultured with MSC was started 3 h after LPS stimulation. The same procedure was performed without LPS stimulation for NM and NM + MSC groups (a). Immunocytochemistry of EYFP stained with anti-GFP antibody (green), Iba-1 (red) and Hoechst 33342 (blue) (scale bar = 50 μm) (b). The expression of Iba-1, GFAP, TUBB3 and Olig2 in microglia normalized to whole brain tissue by qPCR (c). Phase contrast images of 3 lots MSC (scale bar = 100 μm) (d). Cell number (line) and PDL (bar) of 3 lots MSC (e). Surface markers of MSC that positive for CD73, CD105, CD90, HLA-ABC, and CD44, and negative for CD45, CD34, CD11b, CD 19 and HLA–DR (f). Abbreviation: NM: normal medium, PDL: population doubling level.

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