Table 2 Materials for knockdown, protein immunoblotting, and plasmid constructs.
Product | Manufacturer | Catalog no. | Concentration/dilution |
|---|---|---|---|
Hs_ TP53I3_2 FlexiTube siRNA 20 nmol | Qiagen | SI00069636 | 110 pmol in a 24-well plate |
Hs_BRCA1_13 FlexiTube siRNA 20 nmol | Qiagen | SI02654575 | 110 pmol in 24-well plate |
P53AIP1 siRNA (h) 10 µM | Santa Cruz | SC-37459 | 110 pmol in 24-well plate |
Negative control siRNA 20 nmol | Qiagen | 1027310 | 110 pmol in 24-well plate |
PIG3 antibody- mouse (A-5) | Santa Cruz | SC-166664 | 1:1000 overnight incubation |
BRCA1 antibody- mouse (D-9) | Santa Cruz | SC-6954 | 1:200 overnight incubation |
P53AIP1- rabbit antibody | Invitrogen | PA5-20355 | 1:200 overnight incubation |
Beta-actin- rabbit AC-74 antibody | Sigma-Aldrich | A53160-100UL | 1:10,000 overnight incubation |
Donkey anti-rabbit IgG (H + L) Alexa Fluor® 790 | Thermo Fisher | A11374 | 1:10,000; 1 h incubation |
Donkey anti- rabbit IgG (H + L) Alexa Fluor® 680 | Thermo Fisher | A10043 | 1:10,000; 1 h incubation |
Donkey anti-mouse IgG (H + L) Alexa Fluor® 790 | Thermo Fisher | A11371 | 1:10,000; 1 h incubation |
Donkey anti-mouse IgG (H + L) Alexa Fluor® 680 | Abcam | ab175774 | 1:10,000; 1 h incubation |
PIG3 cDNA Clone, human, C-OFPSpark® tag | Sino Biological | HG15531-ACR | N/A |
