Fig. 1: SIRT1 is upregulated in the kidney from UUO modeled mice. | Cell Death Discovery

Fig. 1: SIRT1 is upregulated in the kidney from UUO modeled mice.

From: SIRT1 attenuates renal fibrosis by repressing HIF-2α

Fig. 1

Male wild-type C57BL/6 mice, at 8–10 weeks of age, were subjected to UUO operation. Mice were euthanized 10 days after sham or UUO surgery. a Western blot analysis showed that fibronectin, collagen I (Col I), and a-SMA in the kidney were increased upon UUO surgery. GAPDH was used as a loading control. b Quantification of western blot data as shown in a. c Masson-trichrome staining. Scale bar = 50 μm. d Quantitative analysis for c. e Sirius red staining. Scale bar = 50 μm. f Quantitative analysis for e. g SIRT1 in the kidney was enhanced in UUO-operated mice. Protein levels were analyzed by western blot analysis and GAPDH has used a loading control. h Quantification of western blot data as shown in e. i SIRT1 mRNA levels were increased in the kidney from UUO mice. n = 6. Data are means ± SEM. ***P < 0.001.

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