Fig. 6: RNF31 promoted p53 degradation via the ubiquitin–proteasome pathway. | Cell Death Discovery

Fig. 6: RNF31 promoted p53 degradation via the ubiquitin–proteasome pathway.

From: Taraxasterol acetate targets RNF31 to inhibit RNF31/p53 axis-driven cell proliferation in colorectal cancer

Fig. 6: RNF31 promoted p53 degradation via the ubiquitin–proteasome pathway.

A Western blot showing p53 expression and proteins related to cell growth, such as PCNA, Cyclin D1, and Bcl-xl, in HCT116 and SW480 cells after double depletion of p53 and RNF31 or single depletion of RNF31. B The Ubibrowser website was used to identify targets of RNF31. C A cycloheximide (CHX)-chase experiment was performed to compare the rate of p53 degradation between the RNF31 depletion group and the nonsense depletion group. The left picture shows p53 degradation measured by Western blotting, and the right picture shows the grayscale plot constructed using ImageJ software and GraphPad software. D Western blotting was used to assess the level of p53 when cells were treated with 20 µM MG132 and siRNF31. E Western blotting was conducted to measure the level of ubiquitin when RNF31 expression was depleted.

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