Fig. 3: In vitro simulation of PC12 cell damage due to SCI and the therapeutic effect of using SP2509 to pharmacologically inhibit LSD1. | Cell Death Discovery

Fig. 3: In vitro simulation of PC12 cell damage due to SCI and the therapeutic effect of using SP2509 to pharmacologically inhibit LSD1.

From: Lysine-specific demethylase 1 inhibition enhances autophagy and attenuates early-stage post-spinal cord injury apoptosis

Fig. 3: In vitro simulation of PC12 cell damage due to SCI and the therapeutic effect of using SP2509 to pharmacologically inhibit LSD1.

a CCK-8’s measurement of a quantitative analysis of relative cell viability with various concentrations for treating nocodazole. n = 8. b PC12 cells apoptosis changes in each group were detected based on TUNEL staining. The SCI + SP2509 group had a lower proportion of TUNEL-positive neurons compared with the SCI group. Scale bar = 100 μm. c Counting the TUNEL-positive neurons in the three experimental groups. d Western blot analysis of LSD1, autophagy, and apoptosis proteins following injury in different groups. e Semi-quantitative detection of autophagy and apoptosis protein levels. f Sample images of PC12 cells stained and a quantitative analysis including anti-LC3B and anti-NeuN antibodies in uninjured and injured rats with or without SP2509. DAPI (blue) was used to stain all cell nuclei. Scale bar = 1000 or 250 μm. g Analysis of the amount of LC3 positive differentiated PC12 cells. One-way ANOVA and Tukey’s multiple comparisons test was used to compute p values for mean ± SD of three independent experiments. *p < 0.05; **p < 0.01.

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