Fig. 6: O-GlcNAcylation enhances ferroptosis sensitivity by regulating TFRC. | Cell Death Discovery

Fig. 6: O-GlcNAcylation enhances ferroptosis sensitivity by regulating TFRC.

From: O-GlcNAcylation enhances sensitivity to RSL3-induced ferroptosis via the YAP/TFRC pathway in liver cancer

Fig. 6

A The luciferase activity of reporters containing wild-type TFRC and Del-YAP was measured in Bel-7402 and SMMC-7721 cells with or without YAP knockdown and PUGNAc treatment. B The luciferase activity of the reporters containing TFRC and Del-YAP was measured in Bel-7402 and SMMC-7721 cells with OGT overexpression. C–G OGT was overexpressed in Bel-7402 and SMMC-7721 cells with simultaneous TFRC knockdown (C). The iron concentration in cells treated as indicated was determined (D). Ferroptotic events in cells treated as indicated were evaluated: cell death (E), lipid ROS production (F), and MDA production (G). H A xenograft assay was performed using Bel-7402 cells treated as indicated with or without piperazine erastin treatment. I Schematic abstract of the possible mechanism by which O-GlcNAcylation promotes ferroptosis sensitivity via the YAP/TFRC pathway in HCC cells.

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