Fig. 5: Overexpression of mitochondrial SIRT4 in liver cancer cell lines with stable HBx transfection.

A and B Western blot and qRT-PCR confirm the overexpression of SIRT4 in HepG2-HBx and HepG2.2.15 cells after transfection of SIRT4 expression plasmid. C Fluorescent images showing mitochondrial localization of SIRT4. All error bars show the standard error of the mean (SEM). **p < 0.01 and ***p < 0.001.