Fig. 7: SIRT4 overexpression has anti-proliferative effects on liver cancer cells. | Cell Death Discovery

Fig. 7: SIRT4 overexpression has anti-proliferative effects on liver cancer cells.

From: Tumor suppressive role of mitochondrial sirtuin 4 in induction of G2/M cell cycle arrest and apoptosis in hepatitis B virus-related hepatocellular carcinoma

Fig. 7

A and B Flow cytometry analysis of HepG2-HBx and HepG2.2.15 cells after transfection with SIRT4 plasmid for 24, 48, and 72 h, followed by staining with annexin V-FITC and PI. The percentage of apoptotic cells was increased with time. C and D Flow cytometry analysis of cell cycle distribution indicating SIRT4 overexpression induces cell-cycle arrest at G2/M. E Western blot-analysis of cell-cycle-related proteins expression at 48 h after SIRT4 transfection. F Representative fluorescence images showing the retention of Cyclin B1 in the cytoplasm after SIRT4 overexpression. The quantitative data shown are mean ± SEM of triplicate samples. *p < 0.01 and ***p < 0.0001.

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