Fig. 1: Effects of LPS on HMC3 microglia. | Cell Death Discovery

Fig. 1: Effects of LPS on HMC3 microglia.

From: Human adipose tissue-derived mesenchymal stem cells and their extracellular vesicles modulate lipopolysaccharide activated human microglia

Fig. 1

A Representative phase-contrast images show morphological changes in HMC3 microglia after 24 h incubation with different doses of LPS. Scale bar, 500 μm. B Immunofluorescence staining for CD11b (green fluorescent signal), phalloidin (white), and nuclear DAPI staining (blue) was performed. Scale bar, 49 μm. C Cell viability after 24 h incubation with different doses of LPS was determined by the XTT assay. Values for each treatment are expressed as percentages of the corresponding control (cells untreated). The results shown are the mean ± SEM of triplicates. Double asterisks (**) indicate a significant difference from the LPS-activated group vs control (p < 0.01). D LPS-induced ROS generation in HMC3 microglia cells in response to 24 h LPS exposure was determine using Celigo imaging cytometer. Representative images are shown. E Heat map showing relative cytokines expression. Collected supernatants were subject to cytokine array blots containing 30 different human neurologically relevant proteins. F Representation of relative cytokines expression. The values are shown as mean pixel density.

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