Fig. 2: Human adipose tissue-derived stem cells and extracellular vesicles characterization.

A Morphology of isolated hAD-MSCs after 24 h in culture. Representative images are shown. B Western blotting was used to confirm the abundances of extracellular vesicle marker CD63 in isolated extracellular vesicles (EVs), cell culture supernatants (CM), and cell lysates. Ponceau red staining is representative of total protein content. C Gene map of cytokine array containing 30 different human neurologically relevant proteins; all spots are in duplicate and D cytokine array membranes. E Heat map showing relative cytokines expression. The values are shown as the mean pixel density.