Fig. 1: Fadraciclib reduces metabolic activity and causes apoptosis of AML cell lines.

A The IC50 of fadraciclib at 24, 48 and 72 h in AML cell lines using the rezasurin reduction assay. B Representative flow cytometry plots Annexin V/DAPI apoptosis assay for the OCI-AML3 cell line treated with fadraciclib for 72 h. C Bar charts based on flow cytometry data for the percentage of annexin V-positive cells in AML cell lines treated with fadraciclib for 72 h. D Representative flow cytometry plots and summary bar charts of the percentage of active caspase-3-positive cells in AML cell lines treated with fadraciclib for 72 h. E Representative flow cytometry plots and summary bar charts of cell cycle phase using PI staining of AML cell lines treated with fadraciclib for 72 h. n = 3.Graphs depict mean ± SD (****p < 0.0001). Data were compared using ANOVA.