Fig. 4: CircHIF1A served as a sponge for miR-580-5p in breast cancer cells. | Cell Death Discovery

Fig. 4: CircHIF1A served as a sponge for miR-580-5p in breast cancer cells.

From: Carcinoma-associated fibroblasts derived exosomes modulate breast cancer cell stemness through exonic circHIF1A by miR-580-5p in hypoxic stress

Fig. 4: CircHIF1A served as a sponge for miR-580-5p in breast cancer cells.The alternative text for this image may have been generated using AI.

A Quantitative real-time PCR analysis of circHIF1A expression in lysates of SKBR3 cells with circHIF1A overexpression following biotinylated-circHIF1A pull-down assay. B Biotinylated WT/mutant miR-580 was transfected into SKBR3 cells with circHIF1A overexpression. After streptavidin capture, circHIF1A expression was detected by quantitative real-time PCR. C Luciferase activity in SKBR3 cells co-transfected with luciferase reporters containing circHIF1A sequences with WT or mutated miR-580 binding sites and mimics of miR-580 or controls. D RNA immunoprecipitation (RIP) assay demonstrated that AGO2 could the enrichment of circHIF1A and miR-580 in MDA-MB-231 cells compared to anti-IgG. E Co-localization of circHIF1A with miR-580 in MDA-MB-231 cells. F Quantitative real-time PCR analysis of miR-580 expression in breast cancer tissues. G Negative relationship between circHIF1A and miR-580 in cancer tissues. H, I MiR-580 expression in MDA-MB-231 cells. MDA-MB-231 cells were transfected with circHIF1A siRNA or circHIF1A overexpression, and then total RNA was extracted for real-time RT-PCR of miR-580. *p < 0.05, **p < 0.01.

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