Fig. 6: CircHIF1A regulated cancer cell stemness by CD44. | Cell Death Discovery

Fig. 6: CircHIF1A regulated cancer cell stemness by CD44.

From: Carcinoma-associated fibroblasts derived exosomes modulate breast cancer cell stemness through exonic circHIF1A by miR-580-5p in hypoxic stress

Fig. 6: CircHIF1A regulated cancer cell stemness by CD44.The alternative text for this image may have been generated using AI.

A, B Cell proliferation in breast cancer cells in the presence of hypoxia exosomes from CAFs. MDA-MB-231 and SKBR3 cells were overexpressed CD44 and treated with the exosomes (Exo/H-CAFs/NC, Exo/H-CAFs/sh-circHIF1A, Exo/H-CAFs/NC + CD44, and Exo/H-CAFs/sh-circHIF1A + CD44). The survival ability was assayed by CCK8. C Cell proliferation in breast cancer cells in the presence of hypoxia exosomes from CAFs. MDA-MB-231 and SKBR3 cells were overexpressed CD44 and treated with the exosomes (Exo/H-CAFs/NC, Exo/H-CAFs/sh-circHIF1A, Exo/H-CAFs/NC + CD44, and Exo/H-CAFs/sh-circHIF1A + CD44). The survival ability was assayed by colony formation. D Mammosphere formation in breast cancer cells. MDA-MB-231 and SKBR3 cells were overexpressed CD44 and treated with the exosomes (Exo/H-CAFs/NC, Exo/H-CAFs/sh-circHIF1A, Exo/H-CAFs/NC + CD44, and Exo/H-CAFs/sh-circHIF1A + CD44). The photos of mammospheres were taken under a microscope. E The data were analyzed from (D). F, G The stem cell markers CD44, ALDHA1, OCT4, CD44, and Nanog in breast cancer cells in the presence of H-CAFs-exo (Exo/H-CAFs/NC, Exo/H-CAFs/sh-circHIF1A, Exo/H-CAFs/NC + CD44, and Exo/H-CAFs/sh-circHIF1A + CD44). The expression was assayed by real-time RT-PCR. H The stem cell markers CD44, ALDHA1, OCT4, SOX2, and Nanog in breast cancer cells in the presence of exosomes derived CAFs (Exo/H-CAFs/NC, Exo/H-CAFs/sh-circHIF1A, Exo/H-CAFs/NC + CD44, and Exo/H-CAFs/sh-circHIF1A + CD44). The expression was assayed by western blotting. NC negative control. *p < 0.05, **p < 0.01.

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