Fig. 1: Effect of CCI to neuronal apoptosis and relative expression levels of circ_0000296 and miR-194-5p. | Cell Death Discovery

Fig. 1: Effect of CCI to neuronal apoptosis and relative expression levels of circ_0000296 and miR-194-5p.

From: Effect of circular RNA, mmu_circ_0000296, on neuronal apoptosis in chronic cerebral ischaemia via the miR-194-5p/Runx3/Sirt1 axis

Fig. 1

a Relative expression of circ_0000296 in OGD-induced HT22 cells and HT22 cells in normal with RNase R treatment by quantitative real-time PCR. Data represented as mean ± SD. (n = 5), *P < 0.05 vs. normal HT22 cells with control group. #P < 0.05 vs. normal HT22 cells group with RNase R treatment. b Relative expression of lin-Nufip2 in OGD-induced HT22 cells and HT22 cells in normal with RNase R treatment. Data represented as mean ± SD (n = 5). *P < 0.05 or #P < 0.05 vs. control group. c Flow cytometry analysis of OGD-induced HT22 cells with overexpression of circ_0000296 with annexin V/PI staining. Data represented as mean±SD (n = 3). *P < 0.05 vs. circ_0000296(+)-NC group. d Relative expression of miR-194-5p in OGD-induced HT22 cells and HT22 cells in normal by quantitative real-time PCR. Data represented as mean±SD (n = 5). *P < 0.05 vs. OGD-induced HT22 cells control group. e Flow cytometry analysis of OGD-induced HT22 cells with transfection of miR-194-5p with annexin V/PI staining. Data represented as mean ± SD (n = 3). *P < 0.05 vs. Agomir-194-5p-NC group, #P < 0.05 vs. Antagomir-194-5p-NC group.

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