Fig. 6: Overexpression of circ_0000296 against CCI induced neuronal apoptosis in vivo. | Cell Death Discovery

Fig. 6: Overexpression of circ_0000296 against CCI induced neuronal apoptosis in vivo.

From: Effect of circular RNA, mmu_circ_0000296, on neuronal apoptosis in chronic cerebral ischaemia via the miR-194-5p/Runx3/Sirt1 axis

Fig. 6

a Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labelling (TUNEL) assay (Ă—200, scale bar = 50 μm) sections of the hippocampus CA1 region of Sham (n = 15) and CCI (n = 20) group. Data represented as mean ± SD.*P < 0.05 vs. Sham group. b Flow cytometry analysis of CCI-induced HT22 cells with annexin V/PI staining. Data represented as mean ± SD. (n = 3, each group). *P < 0.05 vs. HT22 in normal group. c TUNEL assay (Ă—200, scale bar = 50 μm) sections of the hippocampus CA1 region of CCI (n = 20), CCI + circ_0000296(+)-NC (n = 20) and CCI + circ_0000296(+) group (n = 15). Data represented as mean ± SD. *P < 0.05 vs. CCI + circ_0000296(+)-NC group. d Experimental schedule to explore the effects of circ_0000296 on CCI-induced neuronal damage in vivo. e The schematic cartoon of the mechanism of circ_0000296/miR-194-5p/Runx3/Sirt1 axis regulating neuronal apoptosis.

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