Fig. 2: PA-Exo induces LX-2 cell activation by transferring miR-107 to LX-2 cells. | Cell Death Discovery

Fig. 2: PA-Exo induces LX-2 cell activation by transferring miR-107 to LX-2 cells.

From: Exosomes secreted by palmitic acid-treated hepatocytes promote LX-2 cell activation by transferring miRNA-107

Fig. 2

A QRT-PCR was performed to detect miR-107 expression in Vehicle-Exo and PA-Exo. B QRT-PCR was performed to determine the transfection efficiency of miR-107I in hepatocytes. The hepatocytes-derived exosomes were divided into Vehicle-Exo, PA-Exo, PA + NCI-Exo, and PA + miR-107I-Exo, then LX-2 cells were cocultured with them for 16 h, respectively. C The hepatocytes were labeled with the lipophilic fluorescent dye DiO (green) and co-incubated with LX-2 cells transfected with mCherry plasmid (red). The laser confocal microscope was used to analyze the intake of exosomes by LX-2 cells (Scale bar = 10 μm). QRT-PCR, CCK-8, and western blot were performed to detect miR-107 expression (D), cell viability (E), and the protein levels of α-SMA and CoL1a1 (F) in LX-2 cells co-incubated with Vehicle-Exo, PA-Exo, PA + NCI-Exo, and PA + miR-107I-Exo, respectively. ****P < 0.0001 vs. Vehicle-Exo; ###P < 0.001 vs. miR-107I-Exo; $$$P < 0.001 vs. PA + NCI-Exo; $$$$P < 0.0001 vs. PA + NCI-Exo. Data were expressed as the mean ± standard deviation (n = 3).

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