Fig. 4: MiR-107 induces LX-2 cell activation through inhibiting DKK1 expression and activating Wnt signaling in LX-2 cells. | Cell Death Discovery

Fig. 4: MiR-107 induces LX-2 cell activation through inhibiting DKK1 expression and activating Wnt signaling in LX-2 cells.

From: Exosomes secreted by palmitic acid-treated hepatocytes promote LX-2 cell activation by transferring miRNA-107

Fig. 4

LX-2 cells were cocultured with hepatocytes-derived exosomes (Vehicle-Exo, PA-Exo, PA + NCI-Exo, and PA + miR-107I-Exo) for 16 h, respectivily. QRT-PCR and western blot were performed to examine the mRNA expression of DKK1 (A) and protein levels of β-catenin, c-myc, and cyclinD1 (B) in LX-2 cells. LX-2 cells were transfected with pcDNA3.1-DKK1 or empty vector, and followed by PA-Exo treatment. CCK-8, and western blot was performed to evaluate LX-2 cell viability (C), the protein levels of α-SMA and CoL1A1 (D), and the protein levels of β-catenin, c-myc, and cyclinD1 (E) in LX-2 cells. **P < 0.01, ****P < 0.0001 vs. Vehicle-Exo; ###P < 0.001, ####P < 0.0001 vs. PA + NCI-Exo; $P < 0.05, $$$P < 0.001, $$$$P < 0.0001 vs. Ctrl; &P < 0.05, &&P < 0.01, &&&P < 0.001, &&&&P < 0.0001 vs. DKK1. Data were expressed as the mean ± standard deviation (n = 3).

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