Fig. 5: PA-Exo induces Th9 cell differentiation and IL-9 expression by transferring miR-107 to CD4+ T cells. | Cell Death Discovery

Fig. 5: PA-Exo induces Th9 cell differentiation and IL-9 expression by transferring miR-107 to CD4+ T cells.

From: Exosomes secreted by palmitic acid-treated hepatocytes promote LX-2 cell activation by transferring miRNA-107

Fig. 5

Naive CD4+ T cells were treated with 10 ng/mL human IL-4, 1 ng/mL human TGF-β, and 10 μg/mL anti-IFN-γ monoclonal antibodies to induce Th9 differentiation, and followed by the coculture with hepatocytes-derived exosomes (Vehicle-Exo, PA-Exo, PA + NCI-Exo, and PA + miR-107I-Exo). A The hepatocytes were labeled with the lipophilic fluorescent dye DiO (green) and co-incubated with CD4+ T cells transfected with mCherry plasmid (red). The laser confocal microscope was used to analyze the intake of exosomes by CD4+ T cells (Scale bar = 10 μm). QRT-PCR and flow cytometry were performed to detect the mRNA expression of miR-107 (B) and IL-9 (C), and Th9 cells proportion (D) in CD4+ T cells. ***P < 0.001, ****P < 0.0001 vs. Vehicle-Exo; ##P < 0.01, ####P < 0.0001 vs. PA + NCI-Exo. Data were expressed as the mean ± standard deviation (n = 3).

Back to article page