Fig. 7: PA-Exo promotes LX-2 cell activation through miR-107-mediated upregulation of IL-9 expression in CD4+ T cells. | Cell Death Discovery

Fig. 7: PA-Exo promotes LX-2 cell activation through miR-107-mediated upregulation of IL-9 expression in CD4+ T cells.

From: Exosomes secreted by palmitic acid-treated hepatocytes promote LX-2 cell activation by transferring miRNA-107

Fig. 7

CD4+ T cells were cocultured with hepatocytes-derived exosomes (Vehicle-Exo, PA-Exo, PA + NCI-Exo, and PA + miR-107I-Exo). Then the culture media were collected and added into culture media of LX-2 cells. CCK-8 and western blot were carried out to evaluate cell viability (A) and the protein levels of α-SMA and CoL1a1 (B) in LX-2 cells. CD4+ T cells were transfected with IL-9 siRNA or scramble control, followed by PA-Exo treatment. CCK-8 and western blot were carried out to evaluate cell viability (C), the protein levels of α-SMA and CoL1a1 (D), and the protein levels of Raf, p-Raf, MEK, p-MEK, ERK, and p-ERK (E) in LX-2 cells. ****P < 0.0001 vs. Vehicle-Exo; ###P < 0.001, ####P < 0.0001 vs. PA + NCI-Exo; $$$P < 0.001, $$$$P < 0.0001 vs. Scramble; &P < 0.05, &&P < 0.01, &&&P < 0.001 vs. IL-9 siRNA. Data were expressed as the mean ± standard deviation (n = 3).

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