Fig. 8: IL-9 promotes LX-2 cell activation through activating Raf/MEK/ERK pathway. | Cell Death Discovery

Fig. 8: IL-9 promotes LX-2 cell activation through activating Raf/MEK/ERK pathway.

From: Exosomes secreted by palmitic acid-treated hepatocytes promote LX-2 cell activation by transferring miRNA-107

Fig. 8

LX-2 cells were transfected with IL-9 overexpression vector or control empty vector. CCK-8 and western blot were carried out to evaluate cell viability (A), the protein levels of α-SMA and CoL1a1 (B), and the protein levels of Raf, p-Raf, MEK, p-MEK, ERK, and p-ERK (C) in LX-2 cells. LX-2 cells were transfected with IL-9 overexpression vector or control empty vector, followed by PD98059 treatment. CCK-8 and western blot were carried out to evaluate cell viability (D), the protein levels of ERK, p-ERK, α-SMA, and CoL1a1 (E) in LX-2 cells. ****P < 0.0001 vs. Ctrl; ###P < 0.001, ####P < 0.0001 vs. IL-9. Data were expressed as the mean ± standard deviation (n = 3).

Back to article page