Fig. 5: The addition of YC-1 and 3-MA inhibited HT-induced autophagy in hypoxia and starvation tumor microenvironment, both of which involved downregulation of the HIF-1α/BNIP3 /Beclin1 signaling pathway. | Cell Death Discovery

Fig. 5: The addition of YC-1 and 3-MA inhibited HT-induced autophagy in hypoxia and starvation tumor microenvironment, both of which involved downregulation of the HIF-1α/BNIP3 /Beclin1 signaling pathway.

From: Combined effects of hyperthermia and chemotherapy on the regulate autophagy of oral squamous cell carcinoma cells under a hypoxic microenvironment

Fig. 5

The mRNA and protein expression of HIF-1α, BNIP3, Beclin1, LC3-II, and p62 in OSCC cells were measured by Western blot and qRT-PCR, which were normalized to β-actin. The results were presented as the mean ± standard deviation of three independent experiments. d: HT + Hy; e: HT + Hy+3-MA; f: HT + Hy+YC-1; g: HT + Hy+YC-1 + 3-MA. HT: hyperthermia; Hy: hypoxia; YC-1: 3-(5′-hydroxymethyl-2′-furyl)−1-benzylindazole; 3-MA: 3-Methyladenine. *p < 0.05 vs. HT + Hy group; #p < 0.05 vs. HT + Hy+3-MA group; &p < 0.05 vs. HT + Hy+YC-1 group; ns no significant difference (p > 0.05).

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