Fig. 2: Depending on oxygen tension, L-DOPA either acts as ROS producer or ROS scavenger. | Cell Death Discovery

Fig. 2: Depending on oxygen tension, L-DOPA either acts as ROS producer or ROS scavenger.

From: Impairment of neuronal mitochondrial function by l-DOPA in the absence of oxygen-dependent auto-oxidation and oxidative cell damage

Fig. 2: Depending on oxygen tension, L-DOPA either acts as ROS producer or ROS scavenger.

Combined NR viability assay and intracellular ROS assay after 6 h of l-DOPA treatment under normoxic (A) and hypoxic (B) conditions including TBHP as a positive control for increased ROS formation and cytotoxicity (n = 5). C Intracellular ROS level after 2 h of l-DOPA treatment (n = 6). D Normalized viability based on NR after 2 h of l-DOPA treatment (200 µM) under normoxic and hypoxic conditions (n = 9). All experiments were done using fully differentiated LUHMES cells, mean, standard error and the single replicates are displayed, significance level were calculated using Welch’s t-test (*p-value < 0.05, **p-value < 0.01, ***p-value < 0.001).

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