Fig. 3: 3-hydroxyanthranic acid inhibits tumor stemness of sorafenib-resistant HCC cells. | Cell Death Discovery

Fig. 3: 3-hydroxyanthranic acid inhibits tumor stemness of sorafenib-resistant HCC cells.

From: 3-hydroxyanthranic acid increases the sensitivity of hepatocellular carcinoma to sorafenib by decreasing tumor cell stemness

Fig. 3

A 3-HAA inhibited cell growth of sorafenib-resistant HCC cells. Cell numbers of HCC cells were counted by cytometry. Data were presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01. B 3-HAA reduced colony formation in sorafenib-resistant SMMC-7721 and PLC8024 cells. Cells were treated with 3-HAA at the indicated dose. *P < 0.05, **P < 0.01. C The ratio of CD44+CD133+ subpopulation was decreased in 3-HAA-treated SMMC-7721 and PLC8024 cells. Cells were treated with 3-HAA at the dose of 100 μM for 48 h. *P < 0.05. D 3-HAA inhibited the expression of stem cell markers in HCC cells. The treating time was 48 h for the dose–response experiment and the dose of 3-HAA was 100 μM for the time-course analysis. E 3-HAA suppressed tumor growth of SMMC-7721 xenografts. The 3-HAA was administrated 100 mg/kg day by intraperitoneal injection for 16 days, five mice were recruited in the control and 3-HAA-treated group. The tumor volumes were presented as mean ± SD. *P < 0.05. F 3-HAA decreased the proportion of CD44+CD133+ subpopulation in SMMC-7721 xenografts. Tumor cells were analyzed by flow cytometry using anti-CD133-PE and anti-CD44-APC. *P < 0.05. G 3-HAA inhibited the expression of stem cell markers in SMMC-7721 xenografts. Bar = 100 μm.

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