Fig. 4: 3-HAA suppressed Wnt signaling by decreasing AKT activity. | Cell Death Discovery

Fig. 4: 3-HAA suppressed Wnt signaling by decreasing AKT activity.

From: 3-hydroxyanthranic acid increases the sensitivity of hepatocellular carcinoma to sorafenib by decreasing tumor cell stemness

Fig. 4

A The KEGG analysis on the upregulated/downregulated genes. The genes whose expression changed more than 2 folds in 3-HAA-treated HCC cells were analyzed. B 3-HAA inhibited GSK3β/β-catenin signaling in SMMC-7721 and PLC8024 cells. The treating time for the dose course experiment was 24 h. The 3-HAA dose for the time course analysis was 100 μM. C The Wnt activator CHIR99021 abolished 3-HAA inhibition on the expression of stem cell markers ALDH, OCT4, and NANOG. The dose of CHIR99021 and 3-HAA was 5 and 100 μM, respectively. Cells were treated for 24 h. D AKT phosphorylation detection in 3-HAA-treated SMMC7721 and PLC8024 cells. The treating time and the 3-HAA dose were the same as above. E SC79 recovered 3-HAA’s inhibition on AKT phosphorylation and GSK3β/β-catenin signaling. The dose of SC79 and 3-HAA were 15 and 100 μM, respectively. Cells were treated for 24 h. F CHIR99021 abolished 3-HAA inhibition on AKT phosphorylation and GSK3β/β-catenin signaling. The dose of CHIR99021 and 3-HAA was 5 and 100 μM, respectively. Cells were treated for 24 h.

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