Fig. 2: Effects of drug-induced expression of POTEF protein in HGrC1 cells. | Cell Death Discovery

Fig. 2: Effects of drug-induced expression of POTEF protein in HGrC1 cells.

From: Primate-specific POTE-actin gene could play a role in human folliculogenesis by controlling the proliferation of granulosa cells

Fig. 2

A Western blotting with anti-Flag antibody. In the HGrC1 cell line with a chemical inducible expression vector for POTEF tagged with Flag (CuO-POTEF-Flag HGrC1), POTEF protein (arrowhead) was detected for 3 days when the cumate chemical solution was present. B Apoptosis of CuO-POTEF-Flag HGrC1 cells treated with or without cumate treatment for 72 h, stained with fluorescein isothiocyanate (FITC)-conjugated annexin V, and analyzed using flow cytometry. Positive control refers to the rate of apoptosis in HGrC1 cells cultured without serum for 72 h. C Analysis of the number of CuO-POTEF-Flag HGrC1 cells counted every 24 h, with or without cumate treatment. D Bromodeoxyuridine (BrdU) proliferation assay of CuO-POTEF-Flag HGrC1 cells with or without cumate treatment. The incorporation of BrdU was measured using an ELISA system. Data are presented as means ± SD. Three independent experiments showed similar results. *P < 0.05 and **P < 0.01 with vs without cumate treatment.

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