Fig. 4: Effects of inhibiting CCT function with siRNA against TCP-1α in HGrC1 cells. | Cell Death Discovery

Fig. 4: Effects of inhibiting CCT function with siRNA against TCP-1α in HGrC1 cells.

From: Primate-specific POTE-actin gene could play a role in human folliculogenesis by controlling the proliferation of granulosa cells

Fig. 4

A Relative protein expression levels were determined with a western blot assay. Transfection of TCP-1α siRNA into HGrC1 cells reduced the expression of TCP-1α; TCP-1θ, another subunit of CCT; and PLK1, a cell growth factor. β-Actin was used as the control. B Alteration in apoptosis of HGrC1 cells with TCP-1α siRNA or control siRNA. Cells were stained with fluorescein isothiocyanate (FITC)-conjugated annexin V and analyzed using flow cytometry. Positive control refers to the rate of apoptosis in HGrC1 cells cultured without serum for 72 h. C Analysis of the number of CuO-POTEF-Flag HGrC1 cells treated with TCP-1α siRNA or control siRNA. Cells were counted every 24 h. D BrdU proliferation assay of CuO-POTEF-Flag HGrC1 cells with TCP-1α siRNA or control siRNA. BrdU incorporation was measured using an ELISA system. Data are presented as means ± SD. Three independent experiments showed similar results. *P < 0.05 TCP-1α siRNA vs control siRNA.

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