Fig. 5: miR-29b-3p targets PTEN.

A The binding site between miR-29b-3p and PTEN in humans and rats was predicted by Targetscan. B The binding relationship between miR-29b-3p and PTEN was verified using a dual-luciferase reporter gene assay. C/D PTEN expression in spinal cord tissues of rats in each group was detected using RT-qPCR and Western blotting. N = 6. Data are expressed as mean ± standard deviation and analyzed using one-way ANOVA or two-way ANOVA, followed by Tukey’s multiple comparisons tests, ***p < 0.001.