Fig. 4: Examination of the phenotypic switch from Ly6Chi to Ly6Clo macrophages regulated by BM-MSCs. | Cell Death Discovery

Fig. 4: Examination of the phenotypic switch from Ly6Chi to Ly6Clo macrophages regulated by BM-MSCs.

From: Mesenchymal stem cells attenuate liver fibrosis by targeting Ly6Chi/lo macrophages through activating the cytokine-paracrine and apoptotic pathways

Fig. 4

A Gating strategy for the isolation of GFP+BM-MSCs from fibrotic livers. B Upregulation of transcriptional expression levels of immunomodulatory factors in FACS-sorted GFP+BM-MSCs from fibrotic livers by quantitative real-time PCR (n = 6). C and D FCM analysis showing the stimulatory effects of IL4 and IL10 on the phenotypic switch of intrahepatic Ly6Chi/Ly6Clo macrophages in a dose-dependent manner (n = 3). E and F FCM analysis showing the inhibitory effects of anti-IL-4 and anti-IL-10 antibodies on IL4/IL10-promoted phenotypic switch of intrahepatic Ly6Chi/Ly6Clo macrophages. IgG2b isotype was used as a control. G and H The anti-IL-4 (0.6 μg/ml) and anti-IL-10 (1 μg/ml) antibodies and 10% ILCM (v/v) were added in BM-MSCs and Ly6Chi macrophages coculture system. FCM analysis showed the direct effect of IL4 and IL10 secreted by BM-MSCs on the phenotypic switch of Ly6Chi/Ly6Clo macrophages. Bars = means ± SD. Statistical evaluation of two groups was performed using an independent Student t-test. Statistical evaluation of multiple groups was performed using one-way ANOVA with posthoc LSD test; n.s. p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.

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