Fig. 4: Combination of SY-1320 and puromycin synergistically induces apoptosis in MCF-7ADR cells.

A MCF-7ADR cells were treated with Puro (1 µg/mL), SY-1320 (2 µM) alone, or both for 24 h, followed by JC-1 staining. Scale bars, 50 µm. B MCF-7ADR cells were treated with Puro (1 µg/mL), SY-1320 (2 µM) alone, or both for 48 h. The cell apoptosis was detected by flow cytometry. Data are presented as mean ± SD of three independent experiments. ***P < 0.001, two-tailed Student’s t test.