Fig. 4: Id2 protects αK40 acetylation by blocking Sirt2-mediated deacetylation. | Cell Death Discovery

Fig. 4: Id2 protects αK40 acetylation by blocking Sirt2-mediated deacetylation.

From: Inhibitor of DNA binding 2 (Id2) mediates microtubule polymerization in the brain by regulating αK40 acetylation of α-tubulin

Fig. 4

A HEK293T cells were transfected as indicated and the cell lysates were subjected to GST pull-down and immunoblotting as indicated. B In vitro deacetylase assay was performed with GFP–Id2 and Flag–Sirt2 proteins. The bar graphs represent the quantification of the acetyl-α-tubulin to α-tubulin ratio. C, D GST–Id2 and GFP–HDAC6 (C) or GFP–αTAT (D) transfected cell lysates were subjected to immunoblotting as indicated. E Primary cultured neurons were co-transfected with GFP–Sirt2 or GFP vector control and RFP–Id2 or RFP vector on DIV 3 and fixed on DIV 5. Neurons were stained with anti-NeuN (blue) and anti-acetylated tubulin (purple). Quantification of fluorescence intensity and axon length is shown at the right. Scale bar, 20 μm. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001. All values are mean ± SEM and the representative images shown are from at least three independent experiments.

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