Fig. 3: m6A reader IGF2BP2 enhanced the stability of lncRNA CASC9. | Cell Death Discovery

Fig. 3: m6A reader IGF2BP2 enhanced the stability of lncRNA CASC9.

From: m6A reader IGF2BP2-stabilized CASC9 accelerates glioblastoma aerobic glycolysis by enhancing HK2 mRNA stability

Fig. 3: m6A reader IGF2BP2 enhanced the stability of lncRNA CASC9.The alternative text for this image may have been generated using AI.

A In the GBM cohort, IGF2BP2 was found to be upregulated according to the GEPIA dataset (http://gepia.cancer-pku.cn/index). B Western blot analysis found the IGF2BP2 expression in U87MG cells with IGF2BP2 overexpression by plasmids transfection. C RNA stability assay demonstrated the remaining lncRNA CASC9 in U87MG cells when treated with Act D. D MeRIP-Seq data by IGV showed the two m6A modification sites in the 3ʹ-UTR of CASC9. E The location of m6A modification sites in the CASC9 gene. F MeRIP-qPCR demonstrated the CASC9 expression in GBM cell lines (U87MG) using anti-m6A antibody. G RIP-qPCR showed the interaction within CASC9 and IGF2BP2 in U87MG cells using anti-IGF2BP2 antibody. Data are presented as the mean ± SD. **P < 0.01.

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