Fig. 5: CASC9/IGF2BP2 complex contributed to the stability of HK2 mRNA. | Cell Death Discovery

Fig. 5: CASC9/IGF2BP2 complex contributed to the stability of HK2 mRNA.

From: m6A reader IGF2BP2-stabilized CASC9 accelerates glioblastoma aerobic glycolysis by enhancing HK2 mRNA stability

Fig. 5: CASC9/IGF2BP2 complex contributed to the stability of HK2 mRNA.The alternative text for this image may have been generated using AI.

A The correlation analysis within the expression of IGF2BP2 and HK2 in the GBM group, which was based on ATGC (http://gepia.cancer-pku.cn/). B MeRIP-Seq indicated that there was a remarkable m6A site in the 3ʹ-UTR of HK2 mRNA. C The m6A site in the 3ʹ-UTR of HK2 mRNA was AGGGACU. D In U251 cells, RT-PCR assay detected the HK2 mRNA levels when transfected with IGF2BP2 overexpression. In U87MG cells, RT-PCR assay detected the HK2 mRNA levels when transfected with CASC9 overexpression. E MeRIP-PCR assay illustrated the HK2 mRNA expression using anti-m6A antibody as compared to Input in U87MG. F RIP-qPCR unveiled the HK2 mRNA level in U251 cells or U87MG cells using IGF2BP2 antibody. G RNA stability assay demonstrated the HK2 mRNA in U251 cells or in U87MG cells when treated with Act D. Data are presented as the mean ± SD. **P < 0.01.

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