Fig. 5: The relationship between P53 and autophagy.
From: Targeting ERK induced cell death and p53/ROS-dependent protective autophagy in colorectal cancer
A–C HCT-116 cells were treated with 10 μM CC90003 for 1, 3, 6, 12 h, and SW620 cells were treated for 12 h. Western blotting was used to detect the level of P53 and ACTIN. D, E Western blotting showed the level of P53, LC3, and ACTIN in shP53 CRC cells after 10 μM CC90003 treatment. F, G Western blotting detected the level of P53, LC3, and ACTIN in CRC cells after treating with CC90003, PFTα, or their combination. H, I FITC/PI staining and flow cytometry analysis of apoptosis in HCT-116 cells treated with CC90003 (10 μM), PFTα (30 μM), or their combination. Annexin-V-positive cells were defined as apoptosis. J, K CRC cells were treated with CC90003 (10 μM), PFTα (30 μM), shP53, or their combination for 24 h, and then the cell viability was detected by the CCK-8 assay.
