Fig. 2: KDM4D inhibition reduces the colony formation, migration, and invasion ability of Caki-1 and 786-O cells.

A Colony formation assays were performed to evaluate the effect of KDM4D inhibition on colony-forming capacity of ccRCC cells. Results have demonstrated that the ability of colony formation in both types of cells was significantly restrained by KDM4D inhibitor. ***pā<ā0.001. B The effects of KDM4D inhibition on cell migration in 786-O and Caki-1 cells were examined by wound healing assays. ***pā<ā0.001. C Images of transwell invasion and migration assays which were used to evaluate the influence of KDM4D inhibition on invasion and migration ability of renal cancer cells. The quantitative analysis was presented below.