Fig. 3: Circ_0004674 could sponge miR-142-5p to function as a ceRNA at the post-transcriptional level in OS progression. | Cell Death Discovery

Fig. 3: Circ_0004674 could sponge miR-142-5p to function as a ceRNA at the post-transcriptional level in OS progression.

From: Doxorubicin-induced novel circRNA_0004674 facilitates osteosarcoma progression and chemoresistance by upregulating MCL1 through miR-142-5p

Fig. 3: Circ_0004674 could sponge miR-142-5p to function as a ceRNA at the post-transcriptional level in OS progression.

A Two miRNAs, hsa-miR-142-5p and hsa-miR-338-3p, which had potential binding sites for circ_0004674 and were simultaneously downregulated in OS tissues and serum, were identified based on three online databases (CircInteractome, dbDEMC and miRCancer). B Predicted potential binding sites for circ_0004674 and miR-142-5p or miR-338-3p. C, D miR-142-5p expression was significantly increased when circ_0004674 was knocked down in the MG63/DXR (or KH-OS/DXR) cell lines, whereas miR-338-3p expression did not obviously change in the two cell lines. E RIP assays showed that circ_0004674 and miR-142-5p could both bind to the Ago2 protein. F Biotin-coupled probe pull-down assay showed that using the biotin-labeled miR-142-5p (miR-142-5p-bio) probe increased the expression of circ_0004674 compared with the control (NC-bio) or miR-142-5p-Mut-Bio probes. G Luciferase activity assay showed that circ_0004674 could combine with miR-142-5p. H Subcellular localization by RNA FISH showed that both circ_0004674 and miR-142-5p were mainly colocalized in the cytoplasm. *P < 0.05, **P < 0.01.

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