Fig. 1: High-dose UVA upregulates the RIPK3 expression in mouse CE. | Cell Death Discovery

Fig. 1: High-dose UVA upregulates the RIPK3 expression in mouse CE.

From: Receptor interacting protein 3 kinase, not 1 kinase, through MLKL-mediated necroptosis is involved in UVA-induced corneal endothelium cell death

Fig. 1

A Flow chart of cornea culture ex vivo and UVA irradiation model. The intensity and time of each UVA irradiation were 20 J/cm2 (25 min 40 seconds every 24 h) for 72 h. B Representative images of Alizarin red S staining in mouse CE from the control and UVA groups (Scale Bar: 20 μm). C Endothelium damage area (%) was analyzed by measuring the Alizarin red S-positive area (n = 5 per group). D LDH release assays of mouse cornea after UVA irradiation (n = 5 per group). E, F Western blot analysis of RIPK3 expression in mouse CE after UVA irradiation (n = 4 per group). G, H Representative immunofluorescence images and analysis of RIPK3 (Red) and DAPI (Blue) localization in mouse CE with control and UVA irradiation. (Scale Bar: 50 μm). The fluorescence intensity of RIPK3 under UVA irradiation was compared based on the control group (n = 5 per group). Statistical significance was analyzed with the unpaired Student’s t-test. **P < 0.01, ***P < 0.001, ****P < 0.0001. All values are expressed as mean ± SD.

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