Fig. 4: Silencing of RIPK3 suppresses cell death via necroptosis pathway in pHCECs. | Cell Death Discovery

Fig. 4: Silencing of RIPK3 suppresses cell death via necroptosis pathway in pHCECs.

From: Receptor interacting protein 3 kinase, not 1 kinase, through MLKL-mediated necroptosis is involved in UVA-induced corneal endothelium cell death

Fig. 4

A Flow chart of pHCECs transfection, culture, and UVA irradiation model. B, C Western blot analysis of RIPK3 in control and asiRNA RIPK3 transfected pHCECs. D Representative images of TUNEL (Green), MLKL (Red), and DAPI (Blue) staining in vehicle, UVA, asiRNA RIPK3 transfected pHCECs (Scale Bar: 20 μm). E, F The TUNEL-positive cells (%) and MLKL fluorescence intensity in normal pHCECs and UVA irradiation pHCECs were quantified (a total of 60–200 cells were selected for statistics in each group, n = 6 per group). Statistical significance was analyzed with the unpaired Student’s t-test. **P < 0.01, ***P < 0.001, ****P < 0.0001. All values are expressed as mean ± SD.

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