Fig. 5: The inhibition of RIPK3 protein expression attenuates cell death after UVA irradiation in transgenic mice CE. | Cell Death Discovery

Fig. 5: The inhibition of RIPK3 protein expression attenuates cell death after UVA irradiation in transgenic mice CE.

From: Receptor interacting protein 3 kinase, not 1 kinase, through MLKL-mediated necroptosis is involved in UVA-induced corneal endothelium cell death

Fig. 5

A Flow chart of Wild-type, RIPK1K45A, RIPK3K51A, RIPK3−/− cornea culture ex vivo and UVA irradiation model. B LDH release assays of transgenic mice CE after UVA irradiation (n = 6 per group). C Alizarin red S staining in transgenic mice CE with control and UVA irradiation (Scale Bar: 20 μm). D Endothelium damage area (%) was analyzed by measuring the Alizarin red S-positive area (n = 5 per group). E Representative immunofluorescence images of TUNEL (Green) and DAPI (Blue) in transgenic mice CE (Scale Bar: 10 μm). F TUNEL-positive cells (%) were quantified and compared among transgenic mice (n = 3 per group). Statistical significance was analyzed with the unpaired Student’s t-test. **P < 0.01, ***P < 0.001, ****P < 0.0001. All values are expressed as mean ± SD.

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